The semi-permeable membrane may have a plurality of pores dimensioned to allow passage of a fluid and prevent passage of the cells and the particles. The device may comprise a semi-permeable membrane, a substrate material constructed and arranged to give structural support to the semi-permeable membrane, the semi-permeable membrane and the substrate material positioned between first and second plates, the first plate defining a first flow chamber adjacent to a first side of the semi-permeable membrane and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber being constructed and arranged to deliver the fluid in a substantially transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber adjacent to a second side of the semi-permeable membrane and comprising a port. In one aspect, there is provided a device for treatment of cells with particles. Aspects and embodiments disclosed herein relate to devices, systems, and methods for the activation of cells. FIELD OF TECHNOLOGYĪspects and embodiments disclosed herein relate to devices, systems, and methods for the transduction of cells. 6, 2017, each of which is incorporated herein by reference in its entirety for all purposes. 62/569,350, titled “APPARATUS FOR EFFICIENT GENETIC MODIFICATION OF CELLS”, filed on Oct. 62/561,164, titled “APPARATUS FOR EFFICIENT GENETIC MODIFICATION OF CELLS”, filed on Sep. 20, 2018, which claims priority under 35 U.S.C. 16/137,478, titled “APPARATUS FOR EFFICIENT GENETIC MODIFICATION OF CELLS,” filed Sep. This application claims priority under 35 U.S.C.
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